Figure 4.

Effects of DCMU and NH₄Cl on NPQ at different temperatures in Symbiodinium OTcH-1. The cells were incubated at 25°C for 30 min in the dark with or without NH₄Cl (100 mm). Next, the cells were incubated at 25°C (A), 33°C (B), or 35°C (C) for 15 min in the dark, and the NPQ development was monitored in the light at 240 µmol photons m⁻² s⁻¹ for 10 min. The relaxation of NPQ was monitored in the dark for another 10 min. DCMU was added to the cells 5 min after the onset of light exposure (arrows). The values are presented as means ± sd of two or three independent experiments.