Figure 3.

Xylan XylT activity of MMs from N. benthamiana leaves coexpressing combinations of wild-type and mutant versions of AoIRX genes from asparagus. Combinations of three wild-type (AoIRX9 or AoIRX9L, AoIRX10, and AoIRX14A or AoIRX14B) and mutated (designated with M) genes were coexpressed in N. benthamiana leaves, and the MMs were extracted for xylan XylT activity analysis. N. benthamiana leaves (co)expressing VENUS and AoIRX9/10/14A were used as negative and positive controls, respectively. A, Xylan XylT products were fractionated by RP-HPLC and detected with a fluorescence detector. The numbers on the plots indicate the degree of polymerization of xylooligosaccharides. B, Xylan XylT was measured using UDP-[¹⁴C]Xyl as the donor and Xyl₅-AA as the acceptor. Values represent means ± se of three biological replicates.