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. 2016 Mar 7;171(1):93–109. doi: 10.1104/pp.15.01919

Figure 4.

Figure 4.

Western blot of a native gel containing solubilized MMs of N. benthamiana leaves coexpressing AoIRX9, AoIRX10, and AoIRX14A. MMs extracted from N. benthamiana leaves coexpressing AoIRX9, AoIRX10, and AoIRX14A were solubilized using 1% (w/v) DDM, separated on a single native gel in three adjacent lanes, and then transferred to a single membrane that was marked for subsequent realignment. The membrane of each lane was then cut and probed with different antibodies against AoIRX9 (left), AoIRX10 (middle), and AoIRX14A (right). The membrane was realigned and subsequently imaged. No monomer bands of AoIRX9, AoIRX10, and AoIRX14A were detected; however, there were some bands detected for AoIRX9 and AoIRX14A between 146 and 242 kD, suggesting the presence of lower oligomers of the protein complex.