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. 2016 Mar 7;171(1):93–109. doi: 10.1104/pp.15.01919

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Figure 9. — IRX9, IRX10, and IRX14A can homodimerize in planta. BiFC was used to test if IRX9, IRX10, and IRX14A can homodimerize in planta. The N- and C-terminal halves of VENUS fused to IRX9 (A), IRX10 (D), and IRX14A (G) were coexpressed in N. benthamiana leaves in the presence of the two other untagged IRX proteins. Fluorescent signal indicates an interaction between the two tagged proteins. A strong punctate signal was observed in all three cases, indicating that IRX9, IRX10, and IRX14A are able to homodimerize. When either IRX9/10/14A-VN (B, E, and H, respectively) or IRX9/10/14A-VC (C, F, and I, respectively) were independently expressed with a noninteracting partner (MUR3-VC/VN; negative controls), no signal was detectable, as expected. Expanded results and positive and negative controls are shown in Supplemental Figures S6 to S8.