Figure 2.

NO evokes short-term [Ca²⁺]_cyt increases. A, SNP was added at 60 and 360 s (n = 5 for each treatment) after the start of the experiment, and [Ca²⁺]_cyt levels were measured for 600 s. B, SNAP was added to provide a final concentration of 150 μm or 0.5% ethanol control was added 60 s after the start of the experiment, and [Ca²⁺]_cyt levels were measured for 600 s (n = 19). C, SNAP to a final concentration of 150 μm was added at 60 s, and 300 μm cPTIO was added at 360 s (n = 21). D, Seedlings were incubated with 300 μm cPTIO for 300 s before the start of the experiment, when 150 μm SNAP was added at 60 s (n = 10). E, SNAP at a final concentration of 150 μm was added 60 s after the start of the experiment, and 50 mm nicotinamide was added 300 s later (n = 20). F, Nicotinamide (50 mm) was added 60 s after the start of the experiment, and 150 μm SNAP was added 300 s later (n = 8). G, SNAP at a final concentration of 150 μm was added 60 s after the start of the experiment, and 50 mm mannitol was added 300 s later (n = 5). H, Seedlings were incubated for 300 s in 1 mm GdCl₃ before the start of the experiment. SNAP at a final concentration of 150 μm was added after 60 s (360 s; n = 13). Arrows indicate the time of each drug addition. Error bars represent se.