βCA2 and βCA4 are both expressed in Arabidopsis leaves. A, Gene fragment of the plus strand of chromosome 5 containing the βCA2 gene (At5g14740) and the upstream gene (At5g14730). The black line labeled pβCA2 indicates the genomic region used to control GUS expression. Blue boxes represent exons, red boxes and arrows represent untranslated regions, and blue and purple lines represent introns and intergenic regions, respectively. Light-colored boxes and lines denote alternative versions of the specified gene. B, Fragment of the antisense strand of chromosome 1 containing the βCA4 gene (At1g70410) and the upstream gene (At1g70420). Black lines labeled pβCA4.1 and pβCA4.2 represent the genomic regions used to control GUS expression in the various βCA4::GUS lines. C, Three-week-old Arabidopsis GUS lines grown in ambient CO2 and constant light showed GUS staining primarily in the leaves of pβCA2::GUS plants, leaves and roots of pβCA4.1::GUS plants, and primarily roots of pβCA4.2::GUS plants.