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. 2016 Mar 10;171(1):359–368. doi: 10.1104/pp.16.00148

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Figure 2. — Detection of in vitro translation products from ORF3s of pri-TAS1a and pri-TAS2. A, Structure of pri-TAS1a and pri-TAS2 derivatives whose ORF3 sequences were partially replaced with FLAG-coding sequences. The changed nucleotides are shown by red characters. Thin and thick underlines represent the miR173 target sites and the FLAG epitope, respectively. B, Detection of FLAG-tagged translation products. After in vitro translation using mdBYL, products were separated using 4% to 12% Bis-Tris NuPAGE gel with MES running buffer. FLAG-tagged products and LUC-myc were detected with anti-FLAG antibody and anti-myc antibody, respectively.