Figure 4.

Polysome analysis of TAS1a- and TAS1c-derived RNAs. Arabidopsis tissue extracts from wild-type (WT) and rdr6 mutant plants were fractionated. RNA was purified from each fraction, separated, and blotted on membranes as described in the legend to Figure 3. RNA blots were first hybridized with TAS1a probe (A). The probe was then stripped, and the same blots were reprobed for TAS1c (B). Total RNA (10 µg) from rdr6 mutant plants was used as a marker for the TAS1a- or TAS1c-related RNAs (the leftmost lane on each gel). Gels stained with ethidium bromide are shown in Supplemental Figure S3. Note that 80S ribosomes were mainly fractionated to the fourth fraction.