Figure 5.

Epistasis analysis of the function of SGS3, RDR6, DCL4, and SDE5. A, Accumulation patterns of TAS2-related RNAs in wild type (WT), single mutants, and sde5 mutation-containing double mutants. Total RNA (20 µg) from each plant was used for northern analysis. RNA blots were hybridized with TAS2 probe. Gels were stained with ethidium bromide, and rRNA was shown as a loading control. B, Detection of tasiR255 derived from TAS1a-c in wild-type, sde5, dcl4, and dcl4 sde5 plants. sRNA (20 µg) was separated on a denaturing 15% polyacrylamide gel and blotted onto a sheet of Hybond-N+ membrane. The blot was probed with tasiR255-AS. The probe was then stripped, and the same blots were reprobed with miR173-AS.