Transient or stable overexpression of Pi02860 enhances P. infestans colonization. A, The graph shows a significant increase (P < 0.001, n = 94) in P. infestans lesion diameter following transient expression in N. benthamiana of GFP-Pi02860 compared with a free GFP control. The Trypan Blue-stained leaf image shows the extent of P. infestans colonization with GFP-Pi02860 or free GFP. B, Transgenic potato lines overexpressing Pi02860 (OE Pi02860-1 and OE Pi02860-2) allow a significant increase (ANOVA, P < 0.002, n = 83) in P. infestans lesion diameter compared with the potato cv E3 control. C, Transgenic potato lines overexpressing Pi02860 allow a significant increase (P < 0.001, n = 64) in P. infestans sporulation compared with the cv E3 control. D, Leaf images taken under UV light show an increase in P. infestans lesions in transgenic potato lines overexpressing Pi02860 compared with the cv E3 control. Lowercase letters on graphs denote statistically significant differences determined by one-way ANOVA, with pairwise comparisons performed with the Holm-Sidak method. Results shown are combinations of at least two independent biological replicates. Error bars show se.