Table II. Linkage analysis of tailored seed mucilage extracted from mum5-1 compared with the wild type.

Wild-type Col-2 and mum5-1 water-extracted mucilage was partially degraded with RGase (3 h) and then precipitated with ethanol. Values (average mol % of neutral sugars) are shown two technical repeats, with variance less than 7% for all samples. Values are scaled to direct neutral sugar analysis (Fig. 4A). tr., Trace.

Sugar and Linkage	Wild-Type Col-2	mum5-1
Rha
t-Rhap	4.33	6.62
2-Rhap	50.99	82.07
2,4-Rhap	4.88	0.81
Ara
t-Araf	0.16	0.10
3-Araf	1.24	0.99
Xyl
t-Xylp	5.78	0.84
4-Xylp	14.67	5.40
2,4-Xylp	4.25	0.77
Man
4,6-Manp	3.30	tr.
Gal
t-Galp	3.69	0.66
3-Galp	0.72	tr.
4,6-Galp	0.99	0.66
Glc
4-Glcp	3.10	1.09
4,6-Glcp	1.00	tr.