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. 2016 Mar 15;171(1):369–379. doi: 10.1104/pp.16.00334

Figure 2.

Figure 2.

CBL10 is present in stamens and pistils throughout flower development. A, RNA was isolated from wild-type flowers collected in three pools representing different stages of development (Smyth et al., 1990) from plants left untreated (Control) or treated with salt (40 mm NaCl). 1, Meristem formation and early flower development (stages 1–11); 2, anthesis (stages 12 and 13); 3, fertilization (stages 14 and 15). AMS, Marker for early flower development; MYB21, marker for late flower development; EF1α, loading control. RNA was also isolated from untreated seedlings (S) as a control. For the CBL10 reaction, bottom band, CBL10; top band, alternatively spliced transcript. B, Expression patterns of the GUS reporter gene under the control of the CBL10 promoter were examined in wild-type flowers at different stages of development (stages 12, 13, and 15, left to right; Smyth et al., 1990). One representative line of 18 total lines is shown. Bar (1 mm, right) shows magnification for all images.