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. 2016 May 2;213(5):733–750. doi: 10.1084/jem.20151724

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© 2016 Jackson et al.

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Figure 6. — B cell–derived IFN-γ is not required for systemic autoimmunity. (A) Percentage IFN-γ⁺ B cells (gated on CD19⁺) in WT, Was^−/−, and B cell–intrinsic Tlr7^−/− and Tlr9^−/− chimeras. (B) Representative flow plots demonstrating expression of the plasma cell marker, CD138, but not the GC B cell marker, GL7, on IFN-γ–producing CD19⁺ B cells. (C and D) Anti-dsDNA (C) and Sm/RNP (D) auto-Ab titers at 12 wk after transplantation. (E–H) Spleen weight (E) and the number of CD4⁺ T cells (F) and CD11b⁺GR1^lo monocyte/macrophages (G), as well as the percentage of PNA⁺FAS⁺ GC B cells (H). Data are representative of two WT (n = 5), two Was^−/− (n = 11), two B cell Ifng^−/− Was^−/− (n = 11), two Was^−/−.Tlr7^−/− (n = 14), and two Was^−/−.Tlr9^−/− (n = 15) chimeras sacrificed 24 wk after transplantation. (A and C–H) Error bars indicate SEM. (A) *, P < 0.05 for each experimental group relative to Was^−/− chimera controls by the Mann-Whitney U-test. (C–H) *, P < 0.05; **, P < 0.01; ****, P < 0.0001, by the Kruskal-Wallis one-way ANOVA.