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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Clin Cancer Res. 2016 Jan 5;22(9):2250–2260. doi: 10.1158/1078-0432.CCR-15-2276

Figure 1. Transient, complete inhibition of PI3K more effectively suppresses cell growth than chronic, partial inhibition.

Figure 1

A) Cells were treated with GDC-0941 for 0, 1, or 24 h, then drug was washed out. Lysates were collected 0, 1, 2, 4, or 8 h post-washout, and analyzed by immunoblotting using the indicated antibodies. B) Cells were serum-starved for 16–24 h, and co-treated with GDC-0941 for 0, 1, or 24 h. After 0 or 1 h of drug washout, cells were stimulated +/− IGF-1 or heregulin (Hrg) for 5 min, then lysed. Lysates were analyzed as in (A). C–D) Cells were treated with 0–1 µM GDC-0941 at time point “0,” and drug was washed out after 0–120 h. In (D), cells were treated with 1 µM fulv. Relative viable cell numbers were measured at 120 h. Data are shown as mean of triplicates + SD.