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. Author manuscript; available in PMC: 2017 May 3.
Published in final edited form as: Anal Chem. 2016 Apr 15;88(9):4780–4787. doi: 10.1021/acs.analchem.6b00124

Table 1.

Performance of Analytical Method to Measure DNA Adducts (per 107 nucleotides).

Adduct Accuracy (CT DNA modified with carcinogens) In vivo (rodent tissue)
Target
Value		 %CV
 Fresh Frozen Liver %CV
 FFPE Liver %CV
Day
1		 Day
2		 Day
3		 inter
-day		 intra
-day		 Day
1		 Day
2		 Day
3		 inter
-day		 intra
-day		 Day
1		 Day
2		 Day
3		 inter
-day		 intra
-day
dG-N2-
B[a]PDE		 Mean 16 17 18 17 12 13 12 12 10 10
SD 1.0 1.5 1.2 1.4 0.81 1.3 1.6 0.48 1.1
%CV 5.8 8.4 7.1 7.3 7.2 12 6.5 11 10 9.9 14 4.7 11 11 13
O6-Me-
dG		 Mean 10 14 13 14 152 147 150 222 199 180
SD 1.4 1.8 2.2 24 9.8 2.3 4.0 26 26
%CV 10 14 16 14 13 16 6.6 2.0 10 9.5 1.8 13 14 10 13
O6-POB-
dG		 Mean 8.2 8.5 8.3 8.2 8.5 8.3 7.1 6.0 6.2
SD 1.2 0.77 0.66 1.2 0.77 0.66 0.47 0.39 0.82
%CV 15 9.0 8.0 11 10 15 9.0 8.0 11 10 6.6 6.6 13 9.1 12
dG-C8-4-
ABP		 Mean 1.9 1.6 1.6 1.5 15 15 15 22 22 22
SD 0.06 0.12 0.18 1.6 1.3 0.69 1.9 3.7 2.4
%CV 3.7 7.2 12 7.9 8.1 10 8.6 4.7 8.3 7.7 8.4 17 11 12 11
dG-C8-
PhIP		 Mean 1.0 1.1 1.2 1.1 0.42 0.41 0.41 0.25 0.30 0.31
SD 0.10 0.06 0.14 0.015 0.013 0.013 0.040 0.028 0.011
%CV 8.7 5.3 13 9.4 9.0 3.4 3.1 3.1 3.2 3.6 16 9.3 3.6 10 14
dA-AL-Ia Mean 5.0 6.9 5.2 5.0 1.8 1.5 1.7 1.5 1.5 1.4
SD 0.19 0.51 0.28 0.15 0.18 0.020 0.057 0.20 0.038
%CV 2.8 9.7 5.6 6.3 19 8.0 12 1.1 8.0 12 3.9 13 2.8 8.2 9.7
a

Kidney DNA of mice treated with AA-I (0.1 mg/kg) was used to perform the accuracy measurement of dA-AL-I.