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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Clin Cancer Res. 2015 Dec 28;22(9):2237–2249. doi: 10.1158/1078-0432.CCR-15-2294

Figure 3. Phenotypic and functional comparison of TIL cultures derived from CM and UM liver metastases.

Figure 3

(A) Mean percentage of CD8+ (filled box) and CD4+ (open box) T cells found in TIL cultures derived from individual CM and UM liver metastases. T cell subset frequency as assessed by flow cytometry and CD3 gating. Box denotes the mean of approximately 24 individual TIL cultures with error bars indicating SEM. (B) Cumulative comparison of CM versus UM derived TIL cultures based upon their percentage of CD8+ and CD4+ T cells. Bars demonstrate the mean with error bars indicating SEM. (C) Autologous tumor reactivity of individual TIL cultures derived from CM and UM liver metastases. Each dot represents the IFN-γ production of a single TIL culture in response to overnight co-culture with autologous tumor digest minus the background cytokine levels of unstimulated TIL and tumor digest alone. Bar represents the mean of approximately 24 TIL cultures. Dashed line marks 100pg/ml of IFN-γ on the y-axis. (D) Cumulative comparison of the anti-tumor reactivity of CM and UM derived TIL cultures based upon production of IFN-γ in response to autologous tumor digest. Bars demonstrate the mean with error bars indicating SEM. Statistical comparisons between CM and UM cohorts were performed with the Student’s t test.