Skip to main content
. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Differentiation. 2015 Nov 3;91(4-5):139–151. doi: 10.1016/j.diff.2015.10.013

Table 2.

Reagents needed for human tissue digest.

  • Fresh human prostate tissue

  • DMEM (GE Healthcare, cat. no SH30249.01)

  • PBS (Corning, cat. no. 21-031-CV)

  • HBSS (GE Healthcare, cat. no. SH30268.01)

  • FBS (Sigma, cat. no. F2442)

  • DMSO (Fisher, cat. no. BP237-700)

  • Trypan Blue (Life Technologies, cat. no. 15250-061)

  • TrypLE Express (Life Technologies, cat. no. 12605-010)

  • RBC lysis buffer (BioLegend, cat. no. 420301)

  • Collagenase type I (Gibco, cat. no. 17100-017)

  • Penicillin-Streptomycin (Corning, cat. no. 30-004-CI)

  • Fungizone (Life Technologies, cat. no. 15290-018)

  • DNase I (Roche, cat. no. 10104159-001)

  • Cell strainers (Corning, cat. nos. 352360, 352340)

  • Rock inhibitor (StemRD, cat. no. y-005)

  • 18-, 20-, 22-G needles (BD Becton Dickinson, cat. nos. 305196, 305175, 305155)

  • 10ml syringe (BD Becton Dickinson, cat. no. 309604)