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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Differentiation. 2015 Nov 3;91(4-5):139–151. doi: 10.1016/j.diff.2015.10.013

Table 7.

Materials and reagents needed for immunohistochemistry:.

  • Cut slides from paraffin-embedded prostate tissue

  • 100%, 95%, 70% ethanol

  • 30% Hydrogen peroxide (Fisher, cat. no. H325-100)

  • Methanol

  • Antigen Retrieval Solution (Dako, cat no. S169984-2)

  • PBS (Corning, cat. no. 21-031-CV)

  • Horse Serum (Vector, cat. no. S-2012)

  • Vectastain universal ABC reagent (Vector, cat. no. PK-6200)

  • DAB (Fisher, cat. no. 34002)

  • Harris Hematoxylin (Sigma, cat. no. HHS128-4L) or hematoxylin of choice

  • 1% acid alcohol

  • 0.3% ammonia water

  • Mounting media such as Cytoseal (Thermo Scientific, cat. no. 8312-4)