. Author manuscript; available in PMC: 2017 Apr 1.

Published in final edited form as: Differentiation. 2015 Nov 3;91(4-5):139–151. doi: 10.1016/j.diff.2015.10.013

Table 8.

Media for Primary Culture of Human Epithelium and Fibroblasts.

Epithelium

RPMI-1640
2.5% DCC-FBS (dextran coated charcoal-stripped and heat-inactivated fetal bovine serum)
Penicillin-Streptomycin (Corning, cat. no. 30-004-CI)
Fungizone (Life Technologies, cat. no. 15290-018)
4μl/ml bovine pituitary extract - (Fisher Sci CB-40123B)
1/100 ITS - insulin-transferrin-selenium (Fisher Sci 50-101-5467)
5μg/ml Cholera toxin (Sigma Aldrich C9903)
10ng/ml EGF (Fisher Sci CB-40052)

Fibroblasts