Fig. 3.
Effect of DTT on activation of the human A2AAR. cAMP accumulation induced by NECA (a) and CGS21680 (b) in CHO cells stably expressing the human A2AAR with and without DTT pretreatment (10 mM, 2 h, 37 °C). Data represent means ± SEM of two independent experiments performed in duplicates. Determined EC50 values for NECA: without DTT: 6.81 ± 1.27 nM; after DTT pretreatment: 606 ± 12 nM***. EC50 values for CGS21680: without DTT: 22.6 ± 3.6 nM; after DTT pretreatment: 119 ± 6 nM**. (Two-tailed t test: ** p < 0.01 and *** p < 0.001)