Fig. 2.

BDNF (100 ng/mL) increases the frequency, but not the amplitude, of spontaneous miniature inhibitory postsynaptic currents (mIPSCs). The miniature IPSC (mIPSC) recordings represented here were performed in CA1 pyramidal cells in whole-cell configuration and in the presence of sodium channel blocker (tetrodotoxin (TTX) 0.5 μM) and a glutamate receptor antagonist (Kynurenic acid, Kyn, 1 mM). a Representative tracings of mIPSCs recorded in the absence (upper trace) and presence (lower trace) of BDNF (100 ng/mL). b Representative average tracings of mIPSCs of two superimposed events in the absence (1) and presence (2) of BDNF (100 ng/mL), from the same cell. c, e Time course changes in mIPSC frequency (c) and amplitude (e) induced by application of BDNF (n = 6). One hundred percent represents the average mIPSC frequency or amplitude recorded for 10 min prior to BDNF application. mIPSC frequency and amplitude changes were quantified by comparing the events from the 10-min period before BDNF application (baseline) to the final 10 min in its presence. d, f The averages of absolute values of frequency (d) or amplitude (f) are shown as individual data obtained in the 10 min in the absence (−) of BDNF and 30–40 min after (+) BDNF administration (n = 6). g, h Averages of the absolute values of rise (g) or decay (h) times are shown as individual data obtained in the 10 min in the absence (−) of BDNF and 30–40 min after (+) BDNF administration (n = 6). Values are mean ± SEM. *p < 0.05 and n.s. p > 0.05 (two-tailed paired Student’s t test)