Fig. 6.

BDNF-mediated effect on mIPSC frequency is dependent on adenosine A_2A receptor activation. The mIPSCs were recorded in the same conditions as described for Fig. 2. During all the recordings, an adenosine A_2A receptor antagonist was further added to the perfusion. a In the upper panel, a representation of mIPSC tracings recorded in the absence (upper trace) and presence (lower trace) of BDNF (10 ng/mL). b Representative average tracings of mIPSCs of two superimposed events in the absence (1) and presence (2) of BDNF (10 ng/mL), from the same cell. c, e Time course changes in mIPSC frequency (c) and amplitude (e) induced by application of BDNF (n = 10). mIPSC frequency and changes were quantified by comparing the events before (baseline) and after BDNF application. d, f Averages of the absolute values of frequency (d) or amplitude (f) are shown as individual data obtained in the absence (−) and presence (+) of BDNF (n = 10). Values are mean ± SEM. n.s. p > 0.05 (two-tailed paired Student’s t test