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. 2016 May 4;6:47. doi: 10.3389/fcimb.2016.00047

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Copyright © 2016 Romero-Espejel, Rodríguez, Chávez-Munguía, Ríos-Castro and Olivares-Trejo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoelectron microscopy of Spbhp-37 protein. S. pneumoniae cellular cultures were incubated in normal Todd-Hewitt Broth or in medium with Hb as only iron source. Then, localization of Spbhp-37 was analyzed by immunoelectron microscopy. (A) Negative control. Bacteria incubated only with the gold-labeled secondary antibodies. (B) Spbhp-37 in normal medium. (C) Spbhp-37 in medium with Hb as only iron source. Arrowheads indicate the location of Spbhp-37 protein in S. pneumoniae. In right is shown the magnification of cell bacteria to compare expression in both growth conditions. (D) Quantitative analysis of Spbhp-37 expression. Gold particles on bacteria growth under normal conditions (TH) and in medium with Hb as only iron source (-Fe+Hb) were counted n = 25. Data represent mean ± SD of three independent experiments. Asterisk indicates a significant difference (p < 0.05).