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. 2016 May 4;6:47. doi: 10.3389/fcimb.2016.00047

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Copyright © 2016 Romero-Espejel, Rodríguez, Chávez-Munguía, Ríos-Castro and Olivares-Trejo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purification of the Spbhp-37 recombinant protein without the GST tag. Spbhp-37-GST recombinant protein was purified by chromatography affinity and the cleavage of GTS tag was performed by the PreScission protease. Then, protein samples were analyzed by 12% SDS-PAGE and western blotting using anti-Spbhp-37 antibodies was tested. (A) Coomassie blue staining. (B) Western blotting with anti-Spbhp-37. (C) Western blotting with anti-GST. Lane 1, Spbhp-37-GST; lane 2, Spbhp-37 without GST; lane 3, GST. Molecular weight markers are indicated at the left side.