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Quantitative analysis of hemoglobin-Sphbp-37 interaction using Biacore assay (Surface Plasmon Resonance). Human hemoglobin was immobilized on sensor chips CM5 (Biacore) and binding curves for Spbhp-37 were expressed in resonance units (RU) as a function of time. The kinetic parameters and concentrations of the analysis are indicated.
