Skip to main content
. 2016 May 4;7:114. doi: 10.3389/fphar.2016.00114

FIGURE 8.

FIGURE 8

Phemindole also shows anti migratory property on TNBC cells. (A) The rate of migration of MDAMB-231 breast cancer cells, on treatment with different concentration of Phemindole (1, 2.5, 5 μM) at 0 and 24 h as determined from bidirectional wound healing assay (left panel). Bar diagram representation of the percentage of migration of MDAMB- 231 cells at 0 and 24 h on treatment with different concentration of Phemindole (1, 2.5, 5 μM) (right panel). (B) Phase contrast images showing the stained MDAMB-231cells as obtained from the trans well migration assay in control and Phemindole treated cells at different concentrations of Phemindole (1, 2.5, 5 μM) (left panel). Percentage of cell migration of MDAMB-231 cells was represented by bar chart under the aforementioned conditions (right panel). (C) Western blot analysis determining the changes in pFAK expression levels of the aforementioned set (upper panel). Quantification of band intensity in respect to loading control was shown in bar diagram (lower panels). Blots are representative of three independent experiments and quantifications are indicated by bar diagram. Values were normalized by intensity of loading control. (D) Immunofluorescence images showing decreased expression of p-FAK in the cytosolic region in response to Phemindole treatment in MDAMB-231 cells. Anti-p-FAK secondary antibody was FITC tagged (green) and nuclei were stained with DAPI (blue) (left panel). Corrected Fluoroscence intensity of p-FAK protein was quantified and represented as bar diagram (right panel). Pictures showed representative cells of each population and were representative of three independent experiments. Values are mean ± SEM of three independent experiments in each case or representative of typical experiment. ∗,∗∗,∗∗∗,# indicate p < 0.05, p < 0.01, p < 0.0005, p > 0.05 respectively versus untreated or control group.