Skip to main content
. 2016 Apr 12;15(3):542–552. doi: 10.1111/acel.12465

Figure 3.

Figure 3

De‐repression of retrotransposons in fat bodies upon fat body‐specific LAM depletion. (A) Similar changes of retrotransposon expression (compared to the 5‐day fat bodies) in old fat bodies or young fat bodies depleted of LAM. The retrotransposons that exhibited altered expression upon aging or LAM depletion were compared with one another. R, Pearson correlation coefficient. (B) Pie chart showing 11 identical de‐repressed (by more than 2 fold) retrotransposons shared between the old fat bodies and LAM‐depleted young fat bodies. (C) A list of up‐regulated retrotransposons (by more than 2 fold) found upon aging and upon LAM depletion in young fat bodies (compared to the control 5‐day old fat bodies). The numbers and color codes show the fold of upregulation. (D) qRT‐PCR analyses of the indicated retrotransposons found to be upregulated by RNA‐seq in young (5 day) fat bodies depleted of LAM (Cg‐gal4/+; tub‐gal80 ts /Lam RNAi). The qRT‐PCR analyses showed that the increased expression of the indicated retrotransposons in the LAM‐depleted young fat bodies (Cg‐gal4/+; tub‐gal80 ts /Lam RNAi) were all significantly up‐regulated as compared to the control young fat bodies depleted of lacZ (Cg‐gal4/+; tub‐gal80 ts / lacZ RNAi). The fold expression change was plotted relative to 5‐day‐old control fat bodies (Cg‐gal4/+; tub‐gal80 ts /+), which was set to 1. Error bars, SEM based on three independent experiments. Student's t test: *P < 0.05, **P < 0.01.