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. 2016 May 4;36(18):4940–4958. doi: 10.1523/JNEUROSCI.3876-15.2016

Figure 2.

Figure 2.

TRIM9 colocalizes and interacts with the netrin-1 receptor DCC in hippocampal neurons. A, Hippocampal neurons at 2 DIV-expressing mCherry-TRIM9 and DCC-pHlourin showing colocalization along the neurite shaft, and at the tips of branches (arrowheads). Graph represents Pearson's colocalization coefficients of DCC to TRIM9 compared with scrambled images of the DCC-pHluorin (n = 7 neurons, mean colocalization = 0.34). B, Western blot of DCC and TRIM9 immunocomplexes precipitated from Trim9+/+ and Trim9−/− E15.5 hippocampal lysate. TRIM9 was coimmunoprecipitated by DCC antibodies, and DCC was coimmunoprecipitated by TRIM9 antibodies in Trim9+/+ but not Trim9−/− samples. C, Western blot of immunoprecipitates shows the splice variants of TRIM9 coimmunoprecipitated by DCC antibodies in Trim9+/+ but not Trim9−/− samples. Goat IgG precipitations shown as a negative control. GAPDH is shown as a loading control.