Table 1.
Genes in study and PCR amplification data.
| Symbol | Name | Gene Bank accession number | Sequence | Annealing temp. (°C) | Number of cycles | Product length (bp) | |
|---|---|---|---|---|---|---|---|
| Rat oligonucleotides | |||||||
| Col1a1 | Collagen, type I, alpha 1 | NM_053304 | Sense antisense |
CCCTCTGTGCCTCAGAAGAACT GCCAGTCTGTTGGTCCATGTAG |
58 | 30 | 234 |
| Col3a1 | Collagen, type III, alpha 1 | NM_032085 | Sense antisense |
AAGAGCGGAGAATACTGGGTTG CAGGATTGCCATAGCTGAACTG |
58 | 30 | 214 |
| Tgfb1 | Transforming growth factor, beta 1 | NM_021578 | Sense antisense |
TGCCAGAACCCCCATTGCTG TCCACCTTGGGCTTGCGACC |
70 | 35 | 700 |
| Ctgf | Connective tissue growth factor | NM_022266 | Sense antisense |
AGAGCTGGGTGTGTGTCCTCC GCAGCAAACACTTCCTCGTGG |
70 | 30 | 547 |
| Acta2 | Actin, alpha 2, smooth muscle, aorta | NM_031004 | Sense antisense |
GTGCCTATCTATGAGGGCTATGCTCTGC CATACTCCTGTTTGCTGATCCACATCTGC |
68 | 35 | 601 |
| Tnf | Tumor necrosis factor | NM_012675 | Sense antisense |
ATGGCATGGATCTCAAAGACAA TCTCCTGGTATGAAGTGGCAAA |
58 | 40 | 150 |
| Adam17 | ADAM metallopeptidase domain 17 | AJ012603 | Sense antisense |
GTGACATGAATGGCAAATGTGA TGGACAAGAATGCTGAAAGGAA |
55 | 40 | 172 |
| Cd36 | CD36 molecule (thrombospondin receptor) | NM_031561 | Sense antisense |
GTCGTATGGTGTGCTGGACATT TGGCTTGACCAGTATGTTGACC |
62 | 35 | 217 |
| Vim | Vimentin | NM_031140 | Sense antisense |
GGATTTCTCTGCCTCTTCCAAA GTCATCGTGGTGCTGAGAAGTC |
62 | 35 | 164 |
| Des | Desmin | NM_022531 | Sense antisense |
TCCGTGCTCAGTATGAGACCAT GCATCAATCTCGCAGGTGTAGG |
62 | 35 | 181 |
| Pdgfb | Platelet-derived growth factor beta polypeptide | NM_031524 | Sense antisense |
AACATGACCCGAGCACATTCT TGGCTTCTTTCTCACAATTTCG |
62 | 35 | 303 |
| Fn1 | Fibronectin 1 | NM_019143 | Sense antisense |
GTGGTCATTTCAGATGCGATTC GGCTCCGAGATACTCTTTCTGC |
62 | 35 | 227 |
| B2m | Beta-2 microglobulin | NM_12512 | Sense antisense |
CGGTGACCGTGATCTTTCTGGT GGTGACGGTTTTGGGCTCCTT |
58 | 30 | 332 |
|
| |||||||
| Rabbit oligonucleotides | |||||||
| TGFB1 | Transforming growth factor, beta 1 | XM_008249704 | Sense antisense |
TCATTTACCGTCACCTGGATTG TGTGTAGATGTTGAGCCCGTTC |
72 | 40 | 229 |
| PDGFB | Platelet-derived growth factor beta polypeptide | XM_008257019 | Sense antisense |
GGTGAGAAAGATCGAGATTGTGC GTGTGCTTGAACTTGTGGTGCT |
62 | 40 | 231 |
| CTGF | Connective tissue growth factor | XM_008263527 | Sense antisense |
GGGCTAAGTTCTGCGGAGTATG CATTGTCCCCAGGACAGTTGTA |
62 | 30 | 162 |
| MMP3 | Matrix metallopeptidase 3 (stromelysin 1, progelatinase) | NM_001082280 | Sense antisense |
TCTCTTCCTTCAGCAGTGGATG TTCCTTATCAGAAATGGCAGCA |
58 | 40 | 186 |
| COL1A1 | Collagen, type I, alpha 1 | XM_008271783 | Sense antisense |
CCTGGGCAGAGAGGAGAAAGAG CCTCACGTCCAGATTCACCAG |
62 | 30 | 157 |
| IGFBP3 | Insulin-like growth factor binding protein 3 | NM_000598 (human) | Sense antisense |
TGCCGTAGAGAAATGGAAGACA GCCCATACTTATCCACACACCA |
72 | 40 | 172 |
| PPARG | Peroxisome proliferator-activated receptor gamma 1 | AY166780 | Sense antisense |
TGATGAATAAAGACGGGGTCCT CCACTGAGAATGATGACGGCTA |
62 | 40 | 187 |
| P4HB | Prolyl 4-hydroxylase, beta polypeptide | NM_001171047 | Sense antisense |
ATGACCAAGTACAAGCCCGAGT GCGTAGAACTCCACGAAGACG |
62 | 30 | 212 |
| COL3A1 | Collagen, type III, alpha 1 | XM_002712333 | Sense antisense |
AAAGAAAGCCCTGAAGCTGATG CCACCAATATCATAGGGTGCAA |
62 | 30 | 195 |
| B2M | Beta-2 microglobulin | XM_002717921 | Sense antisense |
CGCCCCAGATTGATATTGAGTT GATCCCATTTCACTGTCATAGGC |
62 | 30 | 195 |