Table 2.
The morphological observations on the embryos of T. gratilla treated with FSW, highest nongametotoxic concentration of CdCl2 and ZnSO4 during 3 h, 6 h, 9 h, 12 h, and 24 h.
| Time of observation | Treatments | ||
|---|---|---|---|
| FSW (filtered seawater) | Cadmium chloride (CdCl2) | Zinc sulphate (ZnSO4) | |
| 3 hrs | |||
| 2-cell | SCD | SCD | SCD |
| 4-cell | SCD | SCD | SCD |
| 6 hrs | |||
| 8-cell to 16-cell | SCD | SCD, DE, EPC | SCD, DE, EPC |
| 9 hrs | |||
| 32-cell to 64-cell | SCD | ESD, DE | ESD, DE |
| 12 hrs | |||
| Blastula | SCD | NFD, DE | NFD, DE |
| 24 hrs | |||
| Gastrula | SCD | NFD | NFD |
| 2-arm echinopluteus | SCD | NFD | NFD |
| General observation | No abnormalities | Formation of apoptotic bodies, cell fragmentation | Blebbing and deformed embryos |
EPC = embryos with pigmented cells, SCD = successful cell division, ESD = embryo stop dividing, NFD = no further development, and DE = deformed embryos.