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. 2016 Apr 20;2016:8175213. doi: 10.1155/2016/8175213

Table 2.

The morphological observations on the embryos of T. gratilla treated with FSW, highest nongametotoxic concentration of CdCl2 and ZnSO4 during 3 h, 6 h, 9 h, 12 h, and 24 h.

Time of observation Treatments
FSW (filtered seawater) Cadmium chloride (CdCl2) Zinc sulphate (ZnSO4)
3 hrs
 2-cell SCD SCD SCD
 4-cell SCD SCD SCD
6 hrs
 8-cell to 16-cell SCD SCD, DE, EPC SCD, DE, EPC
9 hrs
 32-cell to 64-cell SCD ESD, DE ESD, DE
12 hrs
 Blastula SCD NFD, DE NFD, DE
24 hrs
 Gastrula SCD NFD NFD
 2-arm echinopluteus SCD NFD NFD
 General observation No abnormalities Formation of apoptotic bodies, cell fragmentation Blebbing and deformed embryos

EPC = embryos with pigmented cells, SCD = successful cell division, ESD = embryo stop dividing, NFD = no further development, and DE = deformed embryos.