Table 1.
Oligonucleotides used for plasmid construction.
Oligonucleotide | Restriction site | Sequence |
---|---|---|
P1 | PstI | 5′-ATTCTGCAGTCCGGCTCGTGGCTAAGA-3′ |
P2 | HindIII | 5′-AATAAGCTTTTAGCAGCAGACGACGTCCTC-3′ |
P3 | HindIII | 5′-ATTAAGCTTTTAGGAGTCATGACGGGTAGTG-3′ |
P4 | PstI | 5′-ATTCTGCAGCCGGACGCTGACCTCATC-3′ |
P5 | HindIII | 5′-ATTAAGCTTTTATGGAGGTGGTATCGGAGG-3′ |
P6 | PstI | 5′-AATCTGCAGCGGAGGAAGAGGACGGTT-3′ |