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. 2016 May 4;7:550. doi: 10.3389/fpls.2016.00550

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Copyright © 2016 Negi, Sanagala, Rai and Jain.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Elimination of intrinsic variability in radicle length. (A) Seeds of rice genotype MI48 were germinated in a Petri plate lined with wet filter paper at 28°C for 4 days in dark. (B) Radicle lengths of germinated seedlings were measured by ImageJ program and categorized into different size ranges of 0.5 cm each. Histogram represents per cent seedlings in different size ranges. (C) About 30–40% seedlings falling in the size range of 2.0–3.0 cm were selected and transferred to hydroponic set up for temporal treatment under P+ and P- conditions.