Table 1. Primers used in this study and the results of PCR and sequencing.

Gene	Primer	Sequence (5'-3')	Size (specific species)	Reference	Result (PCR and sequencing)
16s rRNA	F	TCG AGC GCC CGC AAG GGG	905 bp (Brucella spp.)	5	+
	R	AAC CAT AGT GTC TCC ACT AA
BCSP 31	F	TGG CTC GGT TGC CAA TAT CAA	223 bp (B. spp.)	5	+
	R	CGC GCT TGC CTT TCA GGT CTG
Omp2	F	GCG CTA AGG CTG CCG ACG CAA	193 bp (B. spp.)	5	+
	R	ACC AGC CAT TGC GGT CGG TA
Omp31	F	TGA CAG ACT TTT TCG CCG AA	720 bp (B. melitensis)	6	+
	R	TAT GGA TTG CAG CAC CGC
IS711	Ba F	GAC GAA CGG AAT TTT TCC AAT CCC	498 bp (B. abortus)	7	-
	IS711	TGC CGA TCA CTT AAG GGC CTT CAT
	Bm F	AAA TCG CGT CCT TGC TGG TCT GA	731 bp (B. melitensis)	7	+
	IS711	TGC CGA TCA CTT AAG GGC CTT CAT
	Bo F	CGG GTT CTG GCA CCA TCG TCG	976 bp (B. ovis)	7	-
	IS711	TGC CGA TCA CTT AAG GGC CTT CAT
	Bs F	GCG CGG TTT TCT GAA GGT TCA GG	285 bp (B. suis)	7	-
	IS711	TGC CGA TCA CTT AAG GGC CTT CAT
BCSS	F	CCA GAT AGA CCT CTC TGG A	300 bp (B. canis)	8	-
	R	TGG CCT TTT CTG ATC TGT TCT T