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. 2015 Aug 10;35(17):2266–2278. doi: 10.1038/onc.2015.289

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Copyright © 2016 Macmillan Publishers Limited

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Knockdown of NKX6.1 enhances transformation and invasive ability of cancer cells. (a) Expression of NKX6.1 in SiHa cells infected with lentiviruses harboring control shRNA (shCtrl) or NKX6.1 shRNA (shNKX6.1-1, shNKX6.1-2) was analyzed by western blot analysis. β-Actin was used as an internal control. The numbers in the western blots represent the ratios of targets to the internal control. (b–d) Cell proliferation (MTS assay) (b), colony formation (c) and invasion (Matrigel assay) (d) were assessed using SiHa cells. The data are presented as the mean±s.e. from three independent experiments in triplicates (analyzed by unpaired two-tailed t-test). **P<0.01 and ***P<0.001.