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. 2016 May 4;3(4):041802. doi: 10.1117/1.NPh.3.4.041802

Fig. 2.

Fig. 2

Diagram of the correlation strategy using intrinsic landmarks. In addition to the protein of interest, one or more independent, well-described structures are labeled. These should be unambiguously identifiable in electron micrographs as well. After channel alignment of all fluorescent channels only the independent landmarks are used for correlation transformations. Thus, precise and unbiased localization of the protein of interest is achieved.