Fig. 4.

Serial sections through the RVG of a young adult hermaphrodite reveal UNC-7::GFP containing gap junctions and ER. (a) Inverted SEM images of 10 serial 100 nm sections showing neuropil tissue. (b) SIM images of the same regions as in (a). Signals from anti-GFP immunostaining are displayed in red. (c) Overlay of (a) and (b). White and black arrowheads as well as the white arrow point to one gap junction each, as it appears in several consecutive sections of the series. Asterisk marks a signal that is only seen in a single section and is thus not considered a bona fide gap junction. Black arrow marks a pronounced UNC-7::GFP expression in what is very likely ER and not a gap junction. Relevant cell identities are annotated. Names of cells forming a gap junction in the particular section are given in red. Scale bar: 500 nm.