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. 2016 May 4;6:25202. doi: 10.1038/srep25202

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Copyright © 2016, Macmillan Publishers Limited

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(A) De novo production of pleuromutilin (1) and 14-O-acetyl-mutilin (2) in A. oryzae. HPLC traces of untransformed A. oryzae NSAR1 (i), A. oryzae with GGS, Cyclase, P450-1, P450-2, P450-3, ATF, and SDR from C. passeckerianus (ii), and authentic pleuromutilin (iii). All traces were monitored through ELSD (Evaporative Light Scattering Detector). (B) Pleuromutilin and 14-O-acetyl-mutilin titres from A. oryzae transformant strains and C. passeckerianus. The titres are expressed either as mg of compound produced per litre of fungal culture (mg/l) or as mg of compound produced per gram of dry mass of mycelium (mg/g). C. passeckerianus required a total of 10 days between inoculation and harvesting, due to the need for a 5-day seed culture in PVS, followed by another 5-day production culture in CGC. A. oryzae transformants were only grown for 5 days in production culture CMP, which was inoculated directly from plates.