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. 2016 May 4;6:25296. doi: 10.1038/srep25296

Figure 7. The effect on growth on parasites at different temperatures carrying the CBF5 silencing construct and cells overexpressing the snoRNAs guiding modification on H69.

Figure 7

(a). Northern analysis demonstrating the overexpression of the snoRNAs. Cells carrying the vector expressing the four snoRNA genes were induced for four days. RNA was subjected to Northern analysis with the indicated probes. Data are represented as mean ± s.e.m. Experiments were done in triplicate (n = 3), Data are represented as mean ± s.e.m. Experiments were done in triplicate (n = 3), *P < 0.05; **P < 0.01 Student’s t-test (b) (1) Growth of CBF5 silenced cells. Uninduced cells carrying the CBF5 silencing construct (-Tet) were compared with cells induced for silencing at 27 °C and 30 °C. The number of cells in the uninduced culture are plotted in blue and those of induced cultures in red. (2) Growth of cells overexpressing the snoRNAs guiding Ψs on H69. The cells carrying the construct of snoRNAs guiding modification on H69. The designation of the uninduced and induced cells are as in (1). Data are represented as mean ± s.e.m. Experiments were done in triplicate (n = 3).