Figure 3. IL-33 increases TF activity of ECs and EC-derived MPs.

(a,b) HUVECs were incubated for 3, 6, 9 and 24 h in the absence (Co) or presence of rh IL-33 (100 ng/mL), and TF activity was measured at the surface of HUVECs (a) or in MPs isolated from cell culture supernatants (b). (c,d) HUVECs and (e,f) HCAECs were incubated for 6 h without (Co) or with rh IL-33 at 100, 10, 1, and 0.1 ng/mL and TF activity at endothelial cell surface (c,e) or in MPs isolated from the respective cell culture supernatants (d,f) was measured. Values are given in pg/10⁴ cells (a,c,e) or in pg/mL (b,d,f) and represent mean values ± SD of three independent determinations. *p ≤ 0.05 compared to respective control.