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. 2016 May 4;6:25143. doi: 10.1038/srep25143

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Copyright © 2016, Macmillan Publishers Limited

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(a) Mouse bone marrow derived non-adherent cells were cultured in ground (Xg) or subjected to μXg for 24 h. Total RNA isolated from these cells was analyzed for TRAF6 mRNA expression using RT-PCR and (b) the band intensity was measured by ImageJ. The relative band intensity was normalized by β-actin amplification in these cells. Each bar represents the mean ± SD of three independent experiments. *Significant (P < 0.05) difference when compared to Xg. (c) Western blot analysis of TRAF6 expression in mouse bone marrow derived preosteoclast cells under μXg conditions. Cells were cultured in Xg or μXg for 24 h and total cell lysates were analyzed for TRAF6 expression. β-actin expression serves as control.