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. 2016 May 4;6:25143. doi: 10.1038/srep25143

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Copyright © 2016, Macmillan Publishers Limited

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(a) Total RNA isolated from preosteoclast cells cultured in Xg or μXg for 24 h was analyzed for OC-STAMP and DC-STAMP mRNA expression using real-time RT-PCR. (b) Real-time RT-PCR analysis of OC-STAMP and DC-STAMP mRNA expression in preosteoclast cells treated with different concentration of TRAIL (0–200 ng/ml). The mRNA expression was normalized with respect to β-actin amplification. Each bar represents the mean ± SD of three independent experiments. *Significant (P < 0.05) difference when compared to Xg or without TRAIL treatment.