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. 2016 May 4;6:25143. doi: 10.1038/srep25143

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(a) Mouse bone marrow derived non-adherent cells were cultured under μXg for different time-points (0–48 h) and total cell lysates (50 μg of protein) were analyzed for TRAIL expression by western blot. (b) Western blot analysis of TRAIL expression in RANKL treated preosteoclast cells. Mouse bone marrow derived non-adherent cells were treated with different concentration of RANKL (0–50 ng/ml) for 24 h and total cell lysates (100 μg of protein) were analyzed for TRAIL expression. β-actin expression serves as control.