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. 2016 May 4;6:25143. doi: 10.1038/srep25143

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(a) Ground based control RAW 264.7 cells were transfected with empty vector (EV) or TRAIL expression vector (pTRAIL) and over-expression of TRAIL was confirmed after 48 h by western blot. β-actin expression served as control; (b) cultured for OCL formation. (c) TRAP-positive multinucleated OCLs formed at the end of the culture period were scored under microscope. *Significant (P < 0.05) difference when compared to EV.