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. 2016 May 4;6:25302. doi: 10.1038/srep25302

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Copyright © 2016, Macmillan Publishers Limited

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(A) The miR-125b binding site in the 3′UTR region of MMP26. (B) The 3′UTR fragment of MMP26 wild-type containing the binding site of miR-125b was cloned into the immediately downstream of firefly luciferase reporter gene. The putative binding site was mutated to the complementary nucleotides of MMP26 in the mutant-type. (C) Relative luciferase activity in 293T cells co-transfected with miR-125b mimic, miR-125b NC and MMP26-WT or MMP26-MUT vectors. The value in blank group was arbitrarily set at 100% and data in other group were presented as ratios to the blank group. Data were showed as mean±SD. **p < 0.01.