Figure 1. Production and characterization of hPLG.

(A) Platelet-rich plasma obtained from whole blood or apheresis is by ultrasonication and gelled at 37 °C by adding human thrombin. (B) Scanning electron microscopy (SEM) images of fresh fibrin (top), fresh hPLG (middle) and hPLG after 3 days incubation (bottom) at 20,000x magnification. (C,D) Initial concentration and release of GFs in 1, 3 or 5 days of culture by hPLG were determined by ELISA. Data show mean ± SEM. Data are representative of three independent experiments.