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. 2016 May 4;6:25326. doi: 10.1038/srep25326

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(A) ECFC morphology was confirmed by immunofluorescence for specific endothelial markers CD31 and vWF on collagen-coated plastic and hPLG. Total RNA was extracted after 24 h of ECFCs seeding on collagen-coated plastic and hPLG. Expression of endothelial (B) and pro-angiogenic (C) marker genes was assessed by RT-qPCR. The 2^−ΔΔCt analysis method was used to analyze the data with GAPDH used as normaliser. Data show mean ± SEM and are representative of three independent experiments. Statistical significance was tested using Mann-Whitney U-test (*P ≤ 0.05).