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. 2016 May 4;6:25275. doi: 10.1038/srep25275

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Nanoparticle (NP) suspension is added to gold calibrant (Au)/methylcellulose (MC) to make a calibration mix (NP/Cal mix). If necessary, NP/Cal mix is dialyzed in microdialysis straws and extruded before adding heavy metal stain (uranyl acetate). 0.5 μl is loaded onto the plastic coated TEM support grid for counting, sizing and further characterization.