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. 2016 Feb 16;48(5):336–344. doi: 10.1152/physiolgenomics.00129.2015

Fig. 5.

Fig. 5.

Inflammatory gene mRNA levels in the spleen after intraperitoneal LPS injection. Young adult and middle-aged mice were injected with either PBS or LPS (5 mg/kg ip) at times 0 and 24 h (arrows). Spleens were harvested at 0, 3, and 24 h after the 1st LPS injection and 3 h after a 2nd PBS or LPS injection (24+3 h). qRT-PCR was used to evaluated the expression of Ifnγ (A), Il-1β (B), and Tnfα (C). 1 symbol, P < 0.05; 3 symbols, P < 0.01; 3 symbols, P < 0.001; *time point vs. baseline (0 h) in young adult mice; +time point vs. baseline (0 h) in middle-aged mice; #P = 0.059, young adult vs. middle-aged mice at the indicated time point.