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. 2016 Feb 16;48(5):345–360. doi: 10.1152/physiolgenomics.00098.2015

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Fig. 3. — Temporal changes in enzyme activities and Irf4 mRNA levels in polarized macrophages. Control (Media Only), IFN-γ + LPS (M1), IL-4 (M2a), or IL-10 (M2c) conditions were used to achieve macrophage polarization. Nitric oxide (NO) in culture media, arginase activity in cellular protein lysates, and Irf4 mRNA expression were measured prior to (0 h) or at various time points after stimulation. A: NO in the CM and cellular arginase activity. B: Irf4 mRNA. Data reported as means ± SE; #P ≤ 0.002 compared with media only control at corresponding time point; ΨP < 0.001 compared with IFN-γ + LPS condition at corresponding time point; n = 3 biological replicates/stimulating condition/time point in all experiments.