Figure 4. Monoculture NPPs confirm Persister-FACSeq NPPs for positive control system and all promoters tested.

White background indicates positive control system; green background indicates promoters predicted to have distinct normal cell and persister gene expression patterns; red background indicates promoters predicted to not have distinct normal cell and persister gene expression patterns. FACS quantile gating strategies for P_lacZ and Persister-FACSeq are described in Figs 2 and 3, respectively. FACS quantile gating strategies for monoculture P_bolA, P_csiD, P_csiE, P_rssB, P_ydcS, P_eno, and P_frdA are described in Supplementary Fig. S5b. Data are averages of 3 biological replicates; error bars portray s.e.m. NPPs of each quantile from Persister-FACSeq data are shown in Supplementary Fig. S5a. Controls are shown in Supplementary Fig. S6a.